Resource Type
Simple Sequence Repeats (SSR) polymorphism confirmation (L. styraciflua)
Program, Pipeline, Workflow or Method Name
SSR polymorphism pipeline L. styraciflua
Program Version
Data Source
Date Performed
Thursday, August 16, 2018 - 15:16

John Carlson at Pennsylvania State University screened a subset of the above predicted SSRs.


Twenty four American sweetgum trees were sampled from a Pennsylvania State University provenance trial (University Park, PA). DNA was extracted using a modified CTAB method, and DNA quality was assessed on 1% agarose gels. Species primer sets were tested against all collected genotypes within a species with a PCR reaction mixture containing between 4-6 ng DNA per reaction volume, and PCR cycles as follows: 95°C-5 min, 35 cycles of 95°C- 30s, 60°C-45s, 72°C-1 min, and a final 72°C-10 min. Both amplification and polymorphism were detected on 2% agarose gels stained with 0.05% (w/v) ethidium bromide. Polymorphism was estimated based on band width.


Of the 96 tested SSRs, 44 successfully amplified and 28 were found to be polymorphic.

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Biological Samples
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