Ginkgo biloba's transcriptome was assembled from 2 biosamples located on NCBI using bioproject PRJNA271136. FastQC was used to determine overall read quality, detect Illumina primer sequences, and identify any regions that look incorrect. Trimmomatic was then used to remove any Illumina primer sequences, after which RCorrector was used to remove any minor errors that Illumina may have caused. After all the files are cleaned, Trinity was used to assemble the transcriptome, Skewer and STAR were used for refinement and alignment of the transcriptome.