Resource Type
BAC library CMCMBb
Library Description
HindIII BAC library from cultivar Vanuxem.
Library Source
Vanuxem ramet from Greg Miller Mah series
Library Collection Comments
Greg Millers Mah series, Vanuxem, an unknown Chinese whose ramets show high blight resistance. Collected in early fall, 2006 by Greg Miller, sent to Jeff Tomkins. Five grafted ramets of one clone sent to CUGI from Greg Miller, all with the same genotype
Library Construction Comments
Constructed by Eric Fang at Clemson University Genomic Institute. 384 well plant format, host is DH 10B, vector is pIndigoBac536. DNA fragmentation utilized HindIII and primers are T7 and SP6. Selectable marker is chloramphenicol. Average insert size is estimated to be 123 Kb. 73,728 clones are arrayed onto 192 plates, yielding an estimated 11X genome coverage. The library can be placed on 4 filters.
Library Source Confirmation
Marker genotype performed by Jeff Tomkins
Library Availability
Publicly available from Clemson University Genomics Institute. Order a copy of the library, filters, hybridizations, or individual clones. See the Clemson University Genomics Institutes ordering site.
Time Accessioned
Sunday, November 29, 2020 - 13:18
Time Last Modified
Sunday, November 29, 2020 - 13:18
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