Resource Type
BAC library CMCMBd
Library Description
EcoR1 BAC library from cultivar Vanuxem.
Library Source
Vanuxem ramet from Greg Miller Mah series
Library Collection Comments
Greg Millers Mah series, Vanuxem, an unknown Chinese whose ramets show high blight resistance. Collected in early fall, 2006 by Greg Miller, sent to Jeff Tomkins. Five grafted ramets of one clone sent to CUGI from Greg Miller, all with the same genotype
Library Construction Comments
Constructed by Chris Saski at Clemson University Genomic Institute. 384 well plant format, host is DH 10B, vector is pIndigoBac536. DNA fragmentation utilized EcoRI and primers are T7 and SP6. Selectable marker is chloramphenicol. Average insert size is estimated to be 115 Kb. 92,160 clones are arrayed onto 240 plates, yielding an estimated 10X genome coverage. The library can be placed on 5 filters.
Library Source Confirmation
Marker genotype performed by Jeff Tomkins
Library Availability
Time Accessioned
Thursday, September 29, 2022 - 15:49
Time Last Modified
Thursday, September 29, 2022 - 15:49
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