Overview
Library NameTulip Poplar BAC Library (LT__Ba)
Unique NameLT__Ba
OrganismLiriodendron tulipifera (Tulip Poplar)
Typebac_library
HindIII BAC library from clone 108 in the yellow-poplar breeding orchard of the University of Tennessee’s Tree Improvement Program
Properties
Property NameValue
Library sourceYoung floral buds were obtained from clone 108 in the yellow-poplar breeding orchard of the University of Tennessee’s Tree Improvement Program based at Knoxville, Tennessee.
Library construction commentsConstructed by Eric Fang at Clemson University Genomic Institute. 384 well plant format, host is DH 10B, vector is pIndigoBac536. DNA fragmentation utilized HindIII and primers are T7 and SP6. Selectable marker is chloramphenicol. Average insert size is estimated to be 117 Kb. 73,728 clones are arrayed onto 192 plates. The library can be placed on 4 filters. Copies of this library, individual clones, filter sets, and other services are publicly available and may be ordered from CUGI. See the Clemson University Genomics Institutes ordering site.
Library availabilityPublicly available from Clemson University Genomics Institute. Order a copy of the library, filters, hybridizations, or individual clones. See the Clemson University Genomics Institutes ordering site.
Library publication Liang H, Fang EG, Tomkins JP, Luo M, Kudrna D, Kim HR, Arumuganathan K, Zhao S, Leebens-Mack J, Schlarbaum SE, Banks JA, dePamphilis CW, Mandoli DF, Wing RA, Carlson JE. Development of a BAC library for yellow-poplar (Liriodendron tulipifera) and the identification of genes associated with flower development and lignin biosynthesis. Tree Genetics & Genomes (2007) 3:215–225