Resource Type
F. americana
Common Name
White Ash
Organism Image

Other Common Names: american ash
Order: Lamiales
Family: Oleaceae
Chromosome Number: 2n = 46

Cross Reference
Predicted SSRS (genomic)

Publication: Staton M, Best T, Khodwekar S, Owusu S, Xu T, Xu Y, Jennings T, Cronn R, Arumuganathan AK, Coggeshall M, Gailing O. Preliminary Genomic Characterization of Ten Hardwood Tree Species from Multiplexed Low Coverage Whole Genome Sequencing. PloS one. 2015 Dec 23;10(12):e0145031.

In order to develop SSR resources for ten important North American hardwood tree species, we individually barcoded and multiplex sequenced DNA from ten hardwood forest tree species using short reads produced from an Illumina HiSeq. Raw reads can be downloaded from the NCBI short read archive, project SRP021923. Bioinformatic processing included trimming, assembly, SSR identification and primer design, processing details including scripts are available.

Statistics (combination of two sequenced libraries):

Untrimmed Pairs 9,880,130
Untrimmed Bases 1,995,786,260
Reconstructed Fragments 1,912,469
Reconstructed Fragments - Bases 317,644,899
Dinucleotide repeats with primers 875
Trinucleotide repeats with primers 179
Tetranucleotide repeats with primers 25

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Polymorphic SSRS

A subset of SSRs have been selected from green ash transcriptome sequences and screened for polymorphism across 14 ash species:

Noakes AG, Best T, Staton ME, Koch J, Romero-Severson J. Cross amplification of 15 EST-SSR markers in the genus Fraxinus. Conservation Genetics Resources. 2014 Dec 1;6(4):969-70.